Concentration Assay:Article Title: Application of triangular trimers derived from Aβ to create antibodies for immunohistochemical studies and as vaccines against Alzheimer’s disease
Article Snippet: Item Supplier Cat No Storage Concentration for IF and IHC Application Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) Jackson ImmunoResea rch 111-035-144 -80 °C 1: 5000 ELISA 500 ml TMB/E Single Reagent, Blue color, Horseradish Peroxidase Substrate Millipore ES001-500ML 4 °C 50 uL per well ELISA Thioflavine S (Practical Grade) Sigma-Aldrich T1892-25G RT 0.5% in 50% EtOH IF TrueBlackTM Lipofuscin Autofluorescence Quencher GoldBiotechn ology TB-250-1 RT 50 uL per 1 mL of 70% EtOH IF 88% formic acid (Certified ACS) Fisher Chemical, A118P-100 RT 88% IHC and IF Normal goat serum Vector laboratories REF S-1000 4 °C 5% (v/v) IHC and IF Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Invitrogen A-11008 4 °C 1:1000 IF Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 647 Invitrogen A-21244 4 °C 1:1000 IF Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 647 Invitrogen A-21235 4 °C 1:1000 IF Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Invitrogen A-11001 4 °C 1:1000 IF Anti-β-Amyloid, 1-16 [6E10] BioLegend 803015 -20 °C 1:1000 IF 90 Anti-β-Amyloid, 17-24 [4G8] BioLegend 800708 4 °C 1:1000 IF β-Amyloid 1-40, [11A50- B10] BioLegend 805404 4 °C 1:1000 IF Purified anti-β-Amyloid,1- 42 Antibody [12F4] BioLegend 805509 4 °C 1:1000 IF Phospho-Tau (Ser202, Thr205) Monoclonal Antibody (AT8) Invitrogen MN1020 -20 °C 1:1000 IF GFAP Antibody Abcam ab4648 -20 °C 1:1000 IF Anti-IBA-1 (for ICC) Wako 019-19741 -20 °C 1:1500 IF 37% Formaldehyde Fisher Chemical F79-4 RT N/A IF DAPI Fluoromount-G Mounting Medium Southern Biotech 0100-20 4 °C N/A IF VECTASHIELD Vibrance® Antifade Mounting Medium with DAPI Vector laboratories H-1800 N/A N/A IF DPX Mounting Media Sigma-Aldrich SKU 06522x RT N/A IHC Vectastain Elite ABC-HRP Kit Peroxidase Vector Laboratories PK-6100 4 °C Manufacturer’s instructions IHC DAB Substrate Kit, Peroxidase (HRP), with Nickel Vector Laboratories SK-4100 4 °C Manufacturer’s instructions IHC Normal Horse Serum Vector Laboratories REF S-2000 4 °C 5% (v/v) IHC Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated Vector Laboratories BA-1100-1.5 4 °C 1:1000 IHC 1% Cresyl Violet Abcam ab246817 4 °C 0.1% IHC 91
Immunohistochemistry:Article Title: Application of triangular trimers derived from Aβ to create antibodies for immunohistochemical studies and as vaccines against Alzheimer’s disease
Article Snippet: Item Supplier Cat No Storage Concentration for IF and IHC Application Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) Jackson ImmunoResea rch 111-035-144 -80 °C 1: 5000 ELISA 500 ml TMB/E Single Reagent, Blue color, Horseradish Peroxidase Substrate Millipore ES001-500ML 4 °C 50 uL per well ELISA Thioflavine S (Practical Grade) Sigma-Aldrich T1892-25G RT 0.5% in 50% EtOH IF TrueBlackTM Lipofuscin Autofluorescence Quencher GoldBiotechn ology TB-250-1 RT 50 uL per 1 mL of 70% EtOH IF 88% formic acid (Certified ACS) Fisher Chemical, A118P-100 RT 88% IHC and IF Normal goat serum Vector laboratories REF S-1000 4 °C 5% (v/v) IHC and IF Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Invitrogen A-11008 4 °C 1:1000 IF Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 647 Invitrogen A-21244 4 °C 1:1000 IF Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 647 Invitrogen A-21235 4 °C 1:1000 IF Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Invitrogen A-11001 4 °C 1:1000 IF Anti-β-Amyloid, 1-16 [6E10] BioLegend 803015 -20 °C 1:1000 IF 90 Anti-β-Amyloid, 17-24 [4G8] BioLegend 800708 4 °C 1:1000 IF β-Amyloid 1-40, [11A50- B10] BioLegend 805404 4 °C 1:1000 IF Purified anti-β-Amyloid,1- 42 Antibody [12F4] BioLegend 805509 4 °C 1:1000 IF Phospho-Tau (Ser202, Thr205) Monoclonal Antibody (AT8) Invitrogen MN1020 -20 °C 1:1000 IF GFAP Antibody Abcam ab4648 -20 °C 1:1000 IF Anti-IBA-1 (for ICC) Wako 019-19741 -20 °C 1:1500 IF 37% Formaldehyde Fisher Chemical F79-4 RT N/A IF DAPI Fluoromount-G Mounting Medium Southern Biotech 0100-20 4 °C N/A IF VECTASHIELD Vibrance® Antifade Mounting Medium with DAPI Vector laboratories H-1800 N/A N/A IF DPX Mounting Media Sigma-Aldrich SKU 06522x RT N/A IHC Vectastain Elite ABC-HRP Kit Peroxidase Vector Laboratories PK-6100 4 °C Manufacturer’s instructions IHC DAB Substrate Kit, Peroxidase (HRP), with Nickel Vector Laboratories SK-4100 4 °C Manufacturer’s instructions IHC Normal Horse Serum Vector Laboratories REF S-2000 4 °C 5% (v/v) IHC Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated Vector Laboratories BA-1100-1.5 4 °C 1:1000 IHC 1% Cresyl Violet Abcam ab246817 4 °C 0.1% IHC 91
Enzyme-linked Immunosorbent Assay:Article Title: Application of triangular trimers derived from Aβ to create antibodies for immunohistochemical studies and as vaccines against Alzheimer’s disease
Article Snippet: Item Supplier Cat No Storage Concentration for IF and IHC Application Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) Jackson ImmunoResea rch 111-035-144 -80 °C 1: 5000 ELISA 500 ml TMB/E Single Reagent, Blue color, Horseradish Peroxidase Substrate Millipore ES001-500ML 4 °C 50 uL per well ELISA Thioflavine S (Practical Grade) Sigma-Aldrich T1892-25G RT 0.5% in 50% EtOH IF TrueBlackTM Lipofuscin Autofluorescence Quencher GoldBiotechn ology TB-250-1 RT 50 uL per 1 mL of 70% EtOH IF 88% formic acid (Certified ACS) Fisher Chemical, A118P-100 RT 88% IHC and IF Normal goat serum Vector laboratories REF S-1000 4 °C 5% (v/v) IHC and IF Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Invitrogen A-11008 4 °C 1:1000 IF Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 647 Invitrogen A-21244 4 °C 1:1000 IF Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 647 Invitrogen A-21235 4 °C 1:1000 IF Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Invitrogen A-11001 4 °C 1:1000 IF Anti-β-Amyloid, 1-16 [6E10] BioLegend 803015 -20 °C 1:1000 IF 90 Anti-β-Amyloid, 17-24 [4G8] BioLegend 800708 4 °C 1:1000 IF β-Amyloid 1-40, [11A50- B10] BioLegend 805404 4 °C 1:1000 IF Purified anti-β-Amyloid,1- 42 Antibody [12F4] BioLegend 805509 4 °C 1:1000 IF Phospho-Tau (Ser202, Thr205) Monoclonal Antibody (AT8) Invitrogen MN1020 -20 °C 1:1000 IF GFAP Antibody Abcam ab4648 -20 °C 1:1000 IF Anti-IBA-1 (for ICC) Wako 019-19741 -20 °C 1:1500 IF 37% Formaldehyde Fisher Chemical F79-4 RT N/A IF DAPI Fluoromount-G Mounting Medium Southern Biotech 0100-20 4 °C N/A IF VECTASHIELD Vibrance® Antifade Mounting Medium with DAPI Vector laboratories H-1800 N/A N/A IF DPX Mounting Media Sigma-Aldrich SKU 06522x RT N/A IHC Vectastain Elite ABC-HRP Kit Peroxidase Vector Laboratories PK-6100 4 °C Manufacturer’s instructions IHC DAB Substrate Kit, Peroxidase (HRP), with Nickel Vector Laboratories SK-4100 4 °C Manufacturer’s instructions IHC Normal Horse Serum Vector Laboratories REF S-2000 4 °C 5% (v/v) IHC Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated Vector Laboratories BA-1100-1.5 4 °C 1:1000 IHC 1% Cresyl Violet Abcam ab246817 4 °C 0.1% IHC 91
Purification:Article Title: Application of triangular trimers derived from Aβ to create antibodies for immunohistochemical studies and as vaccines against Alzheimer’s disease
Article Snippet: Item Supplier Cat No Storage Concentration for IF and IHC Application Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) Jackson ImmunoResea rch 111-035-144 -80 °C 1: 5000 ELISA 500 ml TMB/E Single Reagent, Blue color, Horseradish Peroxidase Substrate Millipore ES001-500ML 4 °C 50 uL per well ELISA Thioflavine S (Practical Grade) Sigma-Aldrich T1892-25G RT 0.5% in 50% EtOH IF TrueBlackTM Lipofuscin Autofluorescence Quencher GoldBiotechn ology TB-250-1 RT 50 uL per 1 mL of 70% EtOH IF 88% formic acid (Certified ACS) Fisher Chemical, A118P-100 RT 88% IHC and IF Normal goat serum Vector laboratories REF S-1000 4 °C 5% (v/v) IHC and IF Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Invitrogen A-11008 4 °C 1:1000 IF Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 647 Invitrogen A-21244 4 °C 1:1000 IF Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 647 Invitrogen A-21235 4 °C 1:1000 IF Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Invitrogen A-11001 4 °C 1:1000 IF Anti-β-Amyloid, 1-16 [6E10] BioLegend 803015 -20 °C 1:1000 IF 90 Anti-β-Amyloid, 17-24 [4G8] BioLegend 800708 4 °C 1:1000 IF β-Amyloid 1-40, [11A50- B10] BioLegend 805404 4 °C 1:1000 IF Purified anti-β-Amyloid,1- 42 Antibody [12F4] BioLegend 805509 4 °C 1:1000 IF Phospho-Tau (Ser202, Thr205) Monoclonal Antibody (AT8) Invitrogen MN1020 -20 °C 1:1000 IF GFAP Antibody Abcam ab4648 -20 °C 1:1000 IF Anti-IBA-1 (for ICC) Wako 019-19741 -20 °C 1:1500 IF 37% Formaldehyde Fisher Chemical F79-4 RT N/A IF DAPI Fluoromount-G Mounting Medium Southern Biotech 0100-20 4 °C N/A IF VECTASHIELD Vibrance® Antifade Mounting Medium with DAPI Vector laboratories H-1800 N/A N/A IF DPX Mounting Media Sigma-Aldrich SKU 06522x RT N/A IHC Vectastain Elite ABC-HRP Kit Peroxidase Vector Laboratories PK-6100 4 °C Manufacturer’s instructions IHC DAB Substrate Kit, Peroxidase (HRP), with Nickel Vector Laboratories SK-4100 4 °C Manufacturer’s instructions IHC Normal Horse Serum Vector Laboratories REF S-2000 4 °C 5% (v/v) IHC Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated Vector Laboratories BA-1100-1.5 4 °C 1:1000 IHC 1% Cresyl Violet Abcam ab246817 4 °C 0.1% IHC 91
Immunocytochemistry:Article Title: Application of triangular trimers derived from Aβ to create antibodies for immunohistochemical studies and as vaccines against Alzheimer’s disease
Article Snippet: Item Supplier Cat No Storage Concentration for IF and IHC Application Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) Jackson ImmunoResea rch 111-035-144 -80 °C 1: 5000 ELISA 500 ml TMB/E Single Reagent, Blue color, Horseradish Peroxidase Substrate Millipore ES001-500ML 4 °C 50 uL per well ELISA Thioflavine S (Practical Grade) Sigma-Aldrich T1892-25G RT 0.5% in 50% EtOH IF TrueBlackTM Lipofuscin Autofluorescence Quencher GoldBiotechn ology TB-250-1 RT 50 uL per 1 mL of 70% EtOH IF 88% formic acid (Certified ACS) Fisher Chemical, A118P-100 RT 88% IHC and IF Normal goat serum Vector laboratories REF S-1000 4 °C 5% (v/v) IHC and IF Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Invitrogen A-11008 4 °C 1:1000 IF Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 647 Invitrogen A-21244 4 °C 1:1000 IF Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 647 Invitrogen A-21235 4 °C 1:1000 IF Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Invitrogen A-11001 4 °C 1:1000 IF Anti-β-Amyloid, 1-16 [6E10] BioLegend 803015 -20 °C 1:1000 IF 90 Anti-β-Amyloid, 17-24 [4G8] BioLegend 800708 4 °C 1:1000 IF β-Amyloid 1-40, [11A50- B10] BioLegend 805404 4 °C 1:1000 IF Purified anti-β-Amyloid,1- 42 Antibody [12F4] BioLegend 805509 4 °C 1:1000 IF Phospho-Tau (Ser202, Thr205) Monoclonal Antibody (AT8) Invitrogen MN1020 -20 °C 1:1000 IF GFAP Antibody Abcam ab4648 -20 °C 1:1000 IF Anti-IBA-1 (for ICC) Wako 019-19741 -20 °C 1:1500 IF 37% Formaldehyde Fisher Chemical F79-4 RT N/A IF DAPI Fluoromount-G Mounting Medium Southern Biotech 0100-20 4 °C N/A IF VECTASHIELD Vibrance® Antifade Mounting Medium with DAPI Vector laboratories H-1800 N/A N/A IF DPX Mounting Media Sigma-Aldrich SKU 06522x RT N/A IHC Vectastain Elite ABC-HRP Kit Peroxidase Vector Laboratories PK-6100 4 °C Manufacturer’s instructions IHC DAB Substrate Kit, Peroxidase (HRP), with Nickel Vector Laboratories SK-4100 4 °C Manufacturer’s instructions IHC Normal Horse Serum Vector Laboratories REF S-2000 4 °C 5% (v/v) IHC Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated Vector Laboratories BA-1100-1.5 4 °C 1:1000 IHC 1% Cresyl Violet Abcam ab246817 4 °C 0.1% IHC 91
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